Regenerative Research Group

Regenerative Research Group

Longevity
Protocol

A physician-directed annual longevity program integrating three biologic preparation classes within a defined schedule of administration, clinical oversight, and reassessment.

Enter the programPrivate Client Edition | 2026

The Program

A structured approach to proactive longevity care

The Longevity Program is a twelve-month, physician-directed framework for individuals pursuing a structured and proactive approach to long-term health, resilience, and healthy aging.

Three biologic preparation classes are integrated within a defined annual schedule, allowing the supervising physician to select the appropriate protocol, establish administration intervals, monitor clinical considerations, and modify the program as appropriate over time.

Designed around continuity rather than a single intervention, the program provides a structured annual framework while preserving individualized physician judgment throughout the year.

Program at a glance

12
Months
3
Biologic components
2
Protocols
Physician directed

Not intended to diagnose, treat, cure, or prevent disease. No preparation described here is represented as having FDA approval for a longevity indication. Participation is elective, individual results may vary, and all clinical decisions remain under the direction of the supervising physician.

Program Components

Three biologic preparation classes

Preparation characteristics are presented by class, concentration, route, and protocol use.

01

EXM

Cryopreserved perinatal tissue matrix preparation

Route
Intramuscular
Volume
4 mL
Interval
By protocol
02

EXS

Secretome preparation with characterized growth factor content

Route
Intravenous
Volume
By protocol
Interval
By protocol
03

EXO-NEURAL

Extracellular vesicle preparation with higher particle concentration

Route
Intranasal, non-injectable
Volume
1 mL
Interval
By protocol

4 scheduled administration visits

Foundational Protocol

The Foundational Protocol distributes three preparation classes across four scheduled administration visits during the twelve-month program. EXM is administered in Months 1 and 7. EXS is administered quarterly at 6 mL in Months 1, 4, 7, and 10. EXO-NEURAL is administered intranasally in Month 1.

Month of administration

01
EXMEXSEXO-NEURAL
02
03
04
EXS
05
06
07
EXMEXS
08
09
10
EXS
11
12
8 mL
Annual EXM total
2 administrations × 4 mL
24 mL
Annual EXS total
4 administrations × 6 mL
1 mL
Annual EXO-NEURAL total
1 intranasal administration

Month 1 begins with the initial administration rather than the calendar year. Administration dates and intervals may be modified by the supervising physician based on individual clinical considerations.

Not intended to diagnose, treat, cure, or prevent disease. No preparation described here is represented as having FDA approval for a longevity indication. Participation is elective, individual results may vary, and all clinical decisions remain under the direction of the supervising physician.

6 scheduled administration visits

Enhanced Protocol

The Enhanced Protocol increases administration frequency across the twelve-month program. EXM is administered in Months 1, 5, and 9. EXS is administered every other month at 3 mL, for six administrations across the year. EXO-NEURAL is administered intranasally in Months 1 and 7.

Month of administration

01
EXMEXSEXO-NEURAL
02
03
EXS
04
05
EXMEXS
06
07
EXSEXO-NEURAL
08
09
EXMEXS
10
11
EXS
12
12 mL
Annual EXM total
3 administrations × 4 mL
18 mL
Annual EXS total
6 administrations × 3 mL
2 mL
Annual EXO-NEURAL total
2 intranasal administrations

The Enhanced Protocol emphasizes more frequent administration across the year. Protocol selection remains subject to individual physician assessment and clinical discretion.

Not intended to diagnose, treat, cure, or prevent disease. No preparation described here is represented as having FDA approval for a longevity indication. Participation is elective, individual results may vary, and all clinical decisions remain under the direction of the supervising physician.

Scientific Context

Understanding the research informing the program

The biologic preparation classes incorporated into this program are associated with areas of active research involving mesenchymal stromal cells, secreted signaling factors, and extracellular vesicles.

How to read this section

The literature summarized here includes preclinical research, animal models, reviews, and early human studies, much of it in patients with diagnosed conditions rather than healthy adults pursuing longevity-oriented care. The published literature does not evaluate the exact annual schedules described here, in this population, or for this purpose. It is presented as scientific context regarding biological plausibility, observed mechanisms, and reported safety findings within the settings studied — it does not establish safety or effectiveness of these protocols or predict an individual outcome.

Systemic Aging and Resilience

Research into healthy aging increasingly examines cellular senescence, intercellular signaling, oxidative stress, mitochondrial function, and the chronic low-grade inflammatory environment associated with aging, often described as inflammaging.

[01]

World Journal of Stem Cells, 2025

An experimental study evaluating mesenchymal stromal cell-derived extracellular vesicles in cellular and animal models of senescence, including vesicle characterization and in vitro and in vivo assessment of senescence-associated outcomes.

In vitro + animal study
[02]

Frontiers in Aging, 2026

A review of stem cell derived vesicles in aging biology, describing anti-inflammatory signaling across models of arthritis, sepsis, cardiovascular and neurological disease, and the transfer of mitochondrial material between cells under oxidative stress.

Review
[03]

Journal of Extracellular Vesicles, 2024

A systematic review and meta-analysis of human extracellular vesicle-based therapies reported low rates of serious adverse events across included studies, while emphasizing substantial heterogeneity in product manufacturing, study design, and adverse-event reporting.

Systematic review + meta-analysis

Musculoskeletal Health

Osteoarthritis represents one of the more extensively studied musculoskeletal applications within extracellular vesicle and mesenchymal stromal cell research and includes emerging human clinical data.

[04]

Journal of Translational Medicine, 2025

Umbilical cord derived exosomes were tested first in cell culture and a mouse model, then in a randomized, double blind, ascending dose study in patients with knee osteoarthritis. The authors reported no adverse consequences and some degree of change in clinical scores and MRI findings.

Randomized human study
[05]

Journal of Cellular and Molecular Medicine, 2021

Exosomes maintained the cartilage cell phenotype by increasing type two collagen synthesis and reducing inflammation driven senescence and apoptosis, with a specific long noncoding RNA identified as partly responsible.

In vitro + animal model
[06]

International Journal of Molecular Sciences, 2025

Vesicles from three tissue sources were compared for cartilage protective effect. All showed low cytotoxicity and suppressed inflammatory markers, with bone marrow and umbilical cord sources performing more strongly than adipose.

In vitro + ex vivo

Neurological Health

The intranasal route is an area of active neurological research because it has been investigated as a potential means of facilitating nose-to-brain transport through pathways associated with the olfactory and trigeminal systems.

[07]

Stem Cells Translational Medicine, 2020

Vesicles from preconditioned mesenchymal stem cells were given intranasally in a triple transgenic Alzheimer model. The material reached the brain and produced immunomodulatory and neuroprotective effects, with a higher density of dendritic spines in treated animals.

Animal model
[08]

Stem Cells, 2021

A review of preclinical research involving intranasal delivery of mesenchymal stem cell-derived extracellular vesicles for neurological disease, including proposed nose-to-brain transport pathways, brain distribution, and outstanding questions regarding dose and biodistribution.

Review
[09]

International Immunopharmacology, 2021

Intranasally administered mesenchymal stem cell-derived small extracellular vesicles were evaluated in an experimental neurological disease model. Treatment was associated with changes in clinical scores, central nervous system histology, and immunomodulatory responses.

Animal model

Not intended to diagnose, treat, cure, or prevent disease. No preparation described here is represented as having FDA approval for a longevity indication. Participation is elective, individual results may vary, and all clinical decisions remain under the direction of the supervising physician.

Clinical Governance

Eligibility and physician oversight

Participation should follow physician review of the individual's medical history, current health status, baseline clinical information, potential contraindications, concurrent medications or therapies, and any findings requiring additional evaluation.

The protocols described here provide a structured annual framework rather than an individualized medical prescription. Preparation selection, dosing, administration route, scheduling, reassessment, modification, continuation, and discontinuation remain within the clinical judgment of the supervising physician.

Before proceeding, patients should complete an informed consent process addressing potential risks, uncertainties, alternatives, and expected follow-up. Clinical findings or changes in health status may warrant modification or discontinuation of the program at any time.

No specific clinical outcome is represented, guaranteed, or implied by participation in the program.

Physician oversight

All administrations are performed or supervised under the direction of an appropriately licensed physician. The treating physician retains responsibility for determining whether participation is appropriate for the individual patient.

Not intended to diagnose, treat, cure, or prevent disease. No preparation described here is represented as having FDA approval for a longevity indication. Participation is elective, individual results may vary, and all clinical decisions remain under the direction of the supervising physician.

Selected Scientific References

Representative research cited within this program

  1. 01Yang SS, Chen SY, Zhuang WY, et al. Efficacy of extracellular vesicles derived from mesenchymal stromal cells in regulating senescence: in vitro and in vivo insights. World J Stem Cells. 2025;17(10):110445. doi:10.4252/wjsc.v17.i10.110445.
  2. 02Dunstan IK, Anthony DC, Lugarini F, et al. Therapeutic potential of stem cell-derived extracellular vesicles in aging and regeneration. Front Aging. 2026;7:1752530. doi:10.3389/fragi.2026.1752530.
  3. 03Van Delen M, Derdelinckx J, Wouters K, et al. A systematic review and meta-analysis of clinical trials assessing safety and efficacy of human extracellular vesicle-based therapy. J Extracell Vesicles. 2024;13(7):e12458. doi:10.1002/jev2.12458.
  4. 04Wang Y, Kong Y, Du J, et al. Injection of human umbilical cord mesenchymal stem cells exosomes for the treatment of knee osteoarthritis: from preclinical to clinical research. J Transl Med. 2025;23:641. doi:10.1186/s12967-025-06623-y.
  5. 05Jin Y, Xu M, Zhu H, et al. Therapeutic effects of bone marrow mesenchymal stem cells-derived exosomes on osteoarthritis. J Cell Mol Med. 2021;25(19):9281-9294. doi:10.1111/jcmm.16860.
  6. 06Sankaranarayanan J, Kim HK, Kang JY, et al. Comparative efficacy of exosomes derived from different mesenchymal stem cell sources in osteoarthritis models: an in vitro and ex vivo analysis. Int J Mol Sci. 2025;26(12):5447. doi:10.3390/ijms26125447.
  7. 07Losurdo M, Pedrazzoli M, D'Agostino C, et al. Intranasal delivery of mesenchymal stem cell-derived extracellular vesicles exerts immunomodulatory and neuroprotective effects in a 3xTg model of Alzheimer's disease. Stem Cells Transl Med. 2020;9(9):1068-1084. doi:10.1002/sctm.19-0327.
  8. 08Herman S, Fishel I, Offen D. Intranasal delivery of mesenchymal stem cells-derived extracellular vesicles for the treatment of neurological diseases. Stem Cells. 2021;39(12):1589-1600. doi:10.1002/stem.3456.
  9. 09Fathollahi A, Hashemi SM, Haji Molla Hoseini M, et al. Intranasal administration of small extracellular vesicles derived from mesenchymal stem cells ameliorated experimental autoimmune encephalomyelitis. Int Immunopharmacol. 2021;90:107207. doi:10.1016/j.intimp.2020.107207.

References are provided for scientific context and do not constitute evidence supporting the specific program schedules described here.